Effects of incompatibility on protoplast fusion between intra-and inter species in basidiomycete, Pleurotus spp.
1989
Go, S.J. | You, C.H. (Rural Development Administration, Suwon (Korea R.). Agricultural Sciences Institute) | Shin, G.C. (Chungnam National Univ., Taejon (Korea R.). Coll. of Agriculture)
Effects of incompatibility existing between intra-and interspecies in Pleurotus spp. on protoplast fusion, clamp formation of their fusants and fruitbody production were investigated. Protoplast fusion between intra-and interspecies of the fungus was achieved by polyethylene glycol treatment. The fusion frequency between intraspecies was a little higher than that of interspecies. Fusion frequency between interspecies was not correlated with their similarities based on isozyme patterns. In case of protoplast fusion between intra-and interspecies, the fusants from the compatible isolates produced normal fruit bodies, while those from the incompatible isolates did not produce clamp connections and fruit bodies except those of a few isolates presumed as mutants
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