Effects of 2-mercaptoethanol on the protoplast formation and osmotic stabilizers on the protoplast reversion of Pyricularia oryzae cavara
1989
Kim, H.T. | Chung, H.S. (Seoul National Univ., Suwon (Korea R.). Coll. of Agriculture)
The mycelia of Pyricularia oryzae were treated with enzyme solution mixture consisting of Driselase, beta-Glucuronidase, Cellulase, and Macerozyme R-10 for the production of protoplasts. More protoplasts were formed from mycelia of race KJ 101 of P. oryzae than of race KI 315a in the enzyme mixtures. The number of protoplasts decreased in the untreated control three hrs after the enzyme treatment, whereas the number increased in the treatments with 10, 50 and 100 mM 2-mercaptoethanol, respectively. The number of protoplasts increased to reach maximum at five hrs after treatment of 10 mM 2-mercaptoethanol, but the least was found in 200 mM. The protoplasts of P. oryzae in a liquid medium containing 2.5 % yeast extract, and 2 % dextrose reverted to the mycelia after five hrs shaking incubation at 27deg C. Some protoplasts produced yeast-like buds and the buds developed to irregularly shaped chains of cells which protruded a germ-tube-like hypha from the distal cell. Once in a while a germ-tube-like hypha protruded directly from the protoplasts. Except in the first type of reversion, other protoplasts reverted to the normal mycelia. The reversion frequency was highest on PDA with stabilizer of 0.6 M KCl. No reversion of protoplasts occurred on water agar regardless of treatments
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