Studies on the promotion of nitrogen fixation and hydrogen evolution in Azolla imbricata by cast iron
1987
Liang Zhong-Jin | Cheng Shuang-Gi | Mo Hsi-Mu (South China Normal Univ., Guangzhou (China). Dept. of Biology)
Azolla imbricata cultivated in Ca superphosphate solution containing cast iron can endure high and low temperatures. Azolla grown in cast iron pots containing 0.03% (w/v) crude Ca superphosphate had higher yield and chlorophyll content, more Grana lamellae in chloroplast of fern, more photosynthetic lamellae in the symbiont Anabaena azollae, and higher C2H4 reduction and H2 evolution rates than that cultivated in enamel ware containing 0.03% Ca superphosphate and 5 mg/liter or 30 mg/liter ferric citrate. It is much easier to culture green algae such as Ocdogonium, Chaetonema, and Ulothrix in the solution with ferric citrate as the Fe source than in the one with cast iron as Fe source. There is significantly more Fe and Mn in the leaves of Azolla cultivated in solution containing cast iron pieces. Cast iron, probably in the form of a chelate, provides azolla with Fe and Mn constantly.
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