Growth and K uptake of Azolla pinnata under different salt levels
1988
Rajarathinam, K. | Padhya, M.A. (M.S. Univ., Baroda. Baroda (India). Tissue Culture Lab.)
Salt tolerance, growth, N content, acetylene reduction, and K accumulation of Azolla pinnata grown under different K levels (0, 10, 20, 30, 40, and 50 ppm) were studied. The plant was grown on a N-free medium containing 40 ppm Mg, 40 ppm K, 40 ppm Ca, 20 ppm P, 0.5 ppm Mn, 0.2 ppm B, 0.1 ppm Mo, 0.01 ppm Cu, 0.01 ppm Zn, and 2 ppm Fe. Salt concentration in the control medium was 770 ppm. To test salt tolerance, NaCl was added to the medium to make 1000, 1300, 1900, 2500, 3100, and 3700 ppm. Three grams of actively growing azolla plants were inoculated in a plastic tray (40- x 25- x 10- cm) containing 2 liters of test medium. Cultures were incubated at 25 plus or minus 2 deg C under fluorescent light for 16/8 h light/dark cycle. Culture medium was changed once in 3 d. Plants were harvested after 21 d. At lower concentrations of salts (1000 and 1300 ppm), azolla grew as well as the control. Salt levels 1900 ppm and higher significantly reduced biomass production, N content, acetylene reduction activity, and K accumulation. Plants died at 3700 ppm salt level.
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