Screening method for immunochemical simultaneous determination of aflatoxin and ochratoxin A in cereals and feeds
1991
Fukal, L. | Marek, M. (Vysoka Skola Chemickotechnologicka, Prague (CSFR))
An immunochemical method was proposed for simultaneous determination of aflatoxin and ochratoxin A with the use of mixed solutions of the following reagents: standards of both mycotoxins, antiserums against the mycotoxins, and radioligands of 125I-aflatoxin B1 and 125I-ochratoxin A. The result of the analytical procedure is the value of concentration of the aflatowin + ochratoxin A sum in the sample. The procedure needs half the amount of reagents as separate determination of each of the two mycotoxins, and is far less laborious. The proposed simultaneous immunoanalysis is suitable for large-area inspection of grain and feed safety from the viewpoint of aflatoxin and ochratoxin A levels.
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