Residue determination of benomyl in grapes
1993
Wang Minghua | Mao Guoping | Yang Chunlong (Nanjing Agricultural Univ., Jiangsu (China). Dept. of Basic Courses)
A method for residue determination of benomyl in grape with high performance liquid chromatography (HPLC) was developed. The benomyl was quantitatively converted into carbendazim by acid hydrolysis. Neutralized to pH 10-11 with sodium hydroxide, the carbendazim was extracted with ethyl acetate. After due concentration, the extract was analyzed by HPLC with UV detection at 285nm. The average recovery was 100.69%. The minimum detectable level was 0.01 mg/kg. The half life of benomyl deposit in grapes was 9.6 d (in Nanjing) and 18.1 d (in Xi'an), and the postharvest residues were 0.7 mg/kg and 1.19 mg/kg respectively, demonstrating that the benomyl residue amount is safe for consumers
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