Predictive efficiency of an enzymatic method to estimate in vivo organic matter digestibility of a mediterranean natural pasture
1994
Riveros V, Edmundo | Alvarez G, Loreto | Mansilla M, Alberto (Universidad de Chile, Santiago (Chile). Fac. de Ciencias Agrarias y Forestales. Dept. de Produccion Animal)
In order to assess the predictive efficiency of enzymatic methods for estimating the organic matter (OM) in vivo digestibility (Do) of the forage produced by an annual natural pasture from the beginning of the annual regrowth until maturity, twenty samples were taken during the annual life cycle, and subjected to an in vivo digestibility trial. Additional determinations were enzymatic digestibility of OM (De) utilizing two commercial enzymes (Rs and R10), in vitro digestibility according to Tilley and Terry (Di) total, N content, OM, acid detergent lignin (Li), hemicellulose (He), neutral detergent fiber (NDF), acid detergent fiber (ADF), cellulose (Ce) and silica (SI). The results were used to calculate stepwise regression in order to obtain a predictive equation of Do. When the determination of coefficient (R2) was low, the ouliers (points which were farthest from the curve) were eliminated and the R2 improvement was analyzed. The results demonstrated that productive efficiency of the enzymatic method cannot be considered high, the best equation being Do=69.76-0.60 FDN + 1.39 Ce; R2 = 0.62; P = 0.01. A better prediction was obtained when the enzymatic method used the R10 enzyme instead of the RS enzyme. The enzymatic method results better than in vitro technique
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