Isolation and purification of the coconut cadang-cadang viroid (CCCVd) for analytical, biological and molecular characterization
1994
Rodriguez, M.J.B. | Namia, M.T.I.
Several isolation procedures for CCCVd [coconut cadang-cadang viroid] that differ mainly by varying the extraction buffer, nucleic acid precipitant and deproteinizing agent to suit the type of tissues used are described. The degree of purity as determined by the number of steps depended on the purpose of isolating the viroid. For routine rapid indexing of CCCVd, two to three steps were favored. However, for biological and molecular characterization, relatively pure isolates were essential. Conditions to minimize viroid degradation in vivo and in vitro were also determined
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Este registro bibliográfico ha sido proporcionado por University of the Philippines at Los Baños