Respiratory syncytial virus in cattle
1993
Hostnik, P. (Veterinary Faculty, Ljubljana (Slovenia))
On the occasion of an epizooty of respiratory diseases in cattle, the GR-91 bovine respiratory syncytial virus (BRSV) was isolated on the AUBEK cellular line. The isolate was identified by direct immunofluorescence test. To determine specific antibodies, in serologic diagnostics several serologic methods were introduced in laboratory work. These methods included seroneutralizing (SN), indirect immunofluorescence (IIF) and immuno-enzymic tests (ELISA). The IIF test was modified and adjusted to the conditions and requirements of the research study. Serologic studies of the incidence of BRSV in cattle in Slovenia were carried out. It was established that the virus was more widespread among the black and white cattle on large publicly-owned farms than among brown and white-spotted cattle on private farms. An active infection with BRSV was established at the common fattening sites where unvaccinated animals from larger cattle breeding farms were brought to
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