Alga spirulina as a source of gamma-linolenic acid and other valuable chemicals
1990
Morakot Tanticharoen (King Mongut's Inst. of Technology Thonburi, Bangkok (Thailand))
For high value added chemical production from this algae. RandD are being carried out to use Spirulina as a source of phycocyanin and gamma-linolenic acid (GLA). Light intensity and diurnal were found to be strongly affected on the GLA content. Total fatty acid and GLA content of Spirulina cultivated in an outdoor pond during summer 1989 were 5.8 % and 1.6 % of dried weight, respectively. The substituted Pyridazinone Sandoz 9785 inhibited the production of GLA. Attemps have been made to grow Spirulina cultures on media containing various levels of the Sandoz 9785. Several mutants growing in media containing Sandoz 9785 higher than 250 ppm are being selected. Preliminary screening for fatty acid and GLA of these selected mutants gave promising result with approximately 7 % TFA and 2 % GLA. After phycocyanin extraction, ethanol or ethanol/hexane was used to extract GLA from residue. Recovery was 95 % for both TFA and GLA at 40 deg. celsius and 1 hour. Fatty acid extract contained 26 % GLA. Purification using silicic acid column chromatography increased the concentration of GLA in glycolipid fraction up to 43 %.
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