Gangliosides as a possible receptor on the bovine erythrocytes for Theileria sergenti
1955
Watarai, S. (Okayama Univ. (Japan). School of Medicine) | Sugimoto, C. | Onoe, S. | Onuma, M. | Yasuda, T.
To elucidate whether or not gangliosides on the bovine erythrocytes serve as a receptor for Theileria sergenti merozoits, the reactivities of the T. sergenti piroplasms with gangliosides were studied by the liposome agglutination test. The parasites reacted weakly with I-active ganglioside containing N-acetylneuraminic acid (NeuAc) and strongly with I-active ganglioside containing N-glycolylneuraminic acid (NeuGc). However, none of the other gangliosides expressed on the bovine erythrocytes, such as GM3 (NeuAc), GM3 (NeuGc), sialosylparagloboside (SPG) (NeuAc), SPG (NeuGc), I-active ganglioside (NeuAc), and I-active ganglioside (NeuGc), were recognized. After infection with T. sergenti, furthermore, the content of I-active ganglioside (NeuAc) was less (p0.05), and I-active ganglioside (NeuGc) content was much less in the erythrocytes (p0.01), though the contents of other NeuAc-and NeuGc-containing gangliosides did not so vary with T. sergenti infection. These results suggest that the parasites recognize the I-active ganglioside as their receptor and bind preferentially to NeuGc-carrying I-active ganglioside rather than to NeuAc-type in the target cell membranes, and that the reduction of the contents of I-active gangliosides (NeuAc and NeuGc) on the erythrocytes was related to T. sergenti infection
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