Application of RAPD markers in bean breeding
1994
Kelly, James D. | Haley, Scott D. | Afanador Kafury, Lucia | Miklas, P.N. | Stavely, J.R.
Four PCR based RAPD markers tightly linked to three major rust resistance genes in beans have been identified by the bean breeding and genetic group at Michigan State University. The resistance genes, Up-2, Ur-3, B-190 which originate in both Andean and Mesoamerican germplasm would offer resistance to the majority of US rust races when combined in a single genotype. Efforts to pyramid these genes through traditional breeding methods have not been possible because of the epistatic interaction of the genes which makes the task impractical because of the large number of test crosses involved. Indirect selection using the four RAPD markers now offers the opportunity to effectively pyramid all three resistance genes into a single cultivar. Identification of RAPD markers and their potential application in bean breeding is discussed.
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