Improvement of high protein rice by tissue culture technique
1990
Tidarat Srikattanaprom
High protein rice (Oryza sativa L.) improvement by using tissue culture technique was divided into 3 steps. The suitable medium for callus induction from embryos of 4 rice cultivars was namely RD 21, RD 25, PTT 60 and KDML 105. The modified Murashige and Skoog (MS) medium supplemented with 1.5 mg/l, 2,4-dichlorophenoxyacetic acid induced the highest frequency of embryogenic callus. Suitable medium for rice plant regeneration from callus was determined. The modified MS medium supplemented with 1 mg/l, naphthaleneacetic acid and 3 mg/l, kinetin gave the highest frequency of regeneration after the callus was dehydrated for 7 days. Selection of the cell lines resistant to feedback inhibition and the regeneration of cell lines into complete rice plants were performed. Lysine and threonine at the concentrations of 1.25x1/10(exp3) and 1.5x1/10(exp3) M and S-aminoethylcystein (S-AEC) analog of lysine at a concentration of 1x1/10(exp3) M. The resistant cell lines with high protein content could be selected by both kinds of selecting agents. The S-AEC was suitable for the selection of resistant cell lines from PTT 60, while the lysine and threonine at a concentration of 1.25x1/10(exp3) M was appropriate selection agent for RD 25 and KDML 105. Similarly, lysine and threonine were suitable for selection cell lines of RD 21. The cell lines of PTT 60, KDML 105 and RD 25 could not regenerate, the calli were proliferated and formed green spots. The green spots were then developed into the roots. The cell lines of RD 21 could regenerate into 3 rice plants. The plants were transferred onto soil in pots and grown until maturity.
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