Effect of the number of subcultures on anatomical and biochemical characteristics of micropropagated strawberry shoots, in relation to their abnormal flowering
1995
Jemmali, A. | Boxus, P. (Station des Cultures Fruitieres et Maraicheres, Gembloux (Belgium)) | Kevers, C. | Gaspar, T.
Strawberry plants (cv Gorella), when produced after a high number of in vitro multiplication cycles (HNS) produce an abnormally high number of flowers per inflorescence in comparison with conventionally propagated plants or with similar plants produced after a few subcultures (LNS). HNS shoots could be morphologically distinguished from LNS shoots by lack of epicuticular wax, closed stomata, larger parenchymatous chloroplasts with faint thylakoids and absence of intergranar frets, and a higher number of plastoglobuli. HNS shoots, of a greener appearance, contained more chlorophyll and their extracts had a higher peroxidase activity than did those of LNS shoots, at the end of the multiplication cycles. The characteristics of HNS shoots might be hyperhydric symptoms induced by intensive multiplication.
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