Incubation temperature affects changes in cucumber seed proteins and mineral content
1996
Russo, V.M. (United States Department of Agriculture, Lane (USA). Agricultural Research Service, South Central Agricultural Research Service) | Biles, C.L.
Suboptimal soil temperatures retard germination of cucumber (Cucumis sativus L.) seed leading to uneven stands and reduced yield. This project was undertaken to determine how concentration of minerals (N, NO2** (-1) NO3** (-1), P HPO4** (-2), K, SO4** (-2), Ca, Fe, Mg, Mn, and Na) and densitiy of protein bands in seed of the cvs. "Arkansas Little Leaf" and "Earlipik 14" changed during germination and radicle elongation. Seed were incubated in distilled water in petri dishes at 13.9, 15.6 and 20 deg C. Seed were analyzed for mineral content and proteins prior to incubation and then at intervals up to 168 h. At 20 deg C at least 90 % of seed of both cultivars germinated by 48 h. The content of N, P, HPO4** (-2) and SO4** (-2) in "Earlipik 14" seed were significantly less at 13.9 or 15.6 than at 20 deg C. This suggests leakage of minerals occurred at suboptimal temperatures. After 24 h incubation at 20 deg C the density of the 70.1 Kda protein band in gels did not increase, while density of 37.4, 43.4, and 50 Kda protein bands increased in seed of both cultivars. At the other temperatures density of protein bands in gels increased until 120 h. The data suggest that low cucumber germination rates at suboptimal temperatures were related to: 1) leakage of minerals and/or 2) the lack of formation or denaturation of proteins associated with germination and radicle elongation
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