Biochemical characterization of rice (Oryza sativa L.) mutants defective of low in nitrate reduction
1995
Ichii, M. (Kagawa Univ., Miki (Japan). Faculty of Agriculture) | Katagiri, T. | Hasegawa, H. | Yatou, O. | Kirk, D.W. | Wray, J.L.
Biological characterization of two rice mutants, M819 selected on the basis of chlorate resistance, and NR827 selected on the basis of poor growth on nitrate as sole nitrogen source, was performed. Both mutants retained XDH activity and had wild-type levels of Mo-Co activity suggesting that they were not defective in the Mo-Co of NR. M819 had a very low level of NR activity and had lost the nitrate-inducible component of CcR activity seen in the wild-type cultivar Norin 8. Net nitrate uptake by M819 was similar to the wild-type. Despite its poor growth on nitrate as sole nitrogen source NR827 retained 80% of the NR activity, and had lost part of the nitrate-inducible CcR activity, seen in the wild-type cultivar Nipponbare. NiR activity was slightly lower than that found in the wild-type cultivar Nipponbare and nitrate content was very much reduced. However net nitrate uptake by NR827 was similar to the wild-type. From these results we conclude that M819 is defective in the apoprotein of NADH-NR. The nature of the biochemical lesion in NR827 is unclear
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