Variations of Nor chromosomes hidden behind RFLP were exposed by fluorescence in situ hybridization in maize [Zea mays]-Triticum somatic hybrid cell lines
1996
Wang, T.B. (Hirosaki Univ., Aomori (Japan). Faculty of Agriculture) | Niizeki, M. | Harada, T. | Ishikawa, R. | Saito, K.
Fluorescence in situ hybridization (FISH) exposed directly the structure and number changes of Nor chromosomes which failed to be detected by restriction fragment length polymorphisms (RFLP) analysis. Di-Nor isochromosomes were directly evidenced by FISH in a somatic hybrid cell line between Zea mays L. (maize) and F1 hybrid (=tritirigia) of Triticum durum Desf. and Elytrigia intermedia (Host) Nevski (=Agropyron intermedium (Host) P.B.). This was caused by structural rearrangement of Nor chromosomes. The interspecific chromosome interaction of maize and tritirigia in somatic hybrid cells was suggested to disturb the mitosis and result in the misdivision of centromere, which leads to the formation of isochromosomes. Besides, the number of Nor chromosomes was observed among the hybrid cell lines using FISH
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