Phylogenetic relationships between cultivated and wild species of rice as revealed by DNA polymorphisms
1996
Ishii, T. | Nakano, T. | Maeda, H. | Kamijima, O. | Khush, G.S.
To clarify the phylogenetic relationships among A genome species, restriction fragment length polymorphism (RFLP) analyses on chloroplast, mitochondrial, and nuclear genomes and random amplified polymorphic DNA (RAPD) analysis were carried out using 30 accessions (8 Oryza sativa, 6 O. glaberrima, 13 O. perennis, and 3 O. breviligulata). Chloroplast DNA was isolated and digested with two restriction endonucleases. To examine mitochondrial and nuclear DNA, total DNA was digested with two endonucleases and subjected to Southern hybridization using 8 mitochondrial and 12 nuclear DNA specific probes, respectively. From the proportion of common fragments, the genetic distances between 30 accessions were calculated, and dendrograms were constructed. RAPD analysis using 14 primers was also carried out, and a phylogenetic tree was made based on the proportion of common fragments. The differentiations of three genomes are mostly harmonious; however, the magnitude of nuclear genome differentiation is much bigger than the other two. The Oceanian, American, and African forms of O. perennis differentiated greatly from others, whereas the O. perennis Asian form and O. breviligulata formed clusters with O. sativa and O. glaberrima, respectively. This indicates that O. sativa and O. glaberrima had originated from the O. perennis Asian form and O. breviligulata, respectively
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