Synthetic Bacillus thuringiensis (Bt) toxin genes for insect resistance and their rapid bioassay using maize endosperm suspension cultures
1996
Altosaar, I. | Sardana, R. | Dukiandjiev, S. | Giband, M. | Cheng, X.
Two plant codon-optimized genes, CrylA(b) and CrylA(c), each consisting of 1,845 bp, have been synthesized using polymerase chain reaction techniques. A maize ubiquitin promoter was used to rapidly test the synthetic CrylA(c)gene. This construct was tested in a maize endosperm-derived suspension culture system. The use of maize endosperm culture in combination with a maize ubiquitin promoter has been found to be a quick and efficient system for testing the activity of synthetic genes
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