Genomic mapping of Pinus sylvestris (L.) using random amplified polymorphic DNA markers
1995
Yazdani, R. (Swedish Univ. of Agricultural Sciences, Uppsala (Sweden). Dept. of Forest Genetics) | Yeh, F.C. | Rimsha, J.
This paper reports the construction of a high-density Random Amplified Polymorphic DNA (RAPD) map in Pinus sylvestris (L.). Genomic DNA of haploid megagametophytes from seeds originating from a single tree were amplified with 220 random decamer oligonucleotide primers by the polymerase chain reaction. Two hundred and ninety-eight RAPDs with fragment sizes ranging between 250 and 2500 base pairs were found segregating at 104 random decamer oligonucleotide primers. Of these, 16 (5 %) were excluded from mapping because they did not confirm to 1:1 Mendelian segregation. Two hundred and sixty-one of the remaining 282 RAPDs formed 14 linkage groups for a total distance of 2,638.6 cM. Four of the 21 RAPDs that were not mapped could not be placed with the main linkage groups. The remaining 17 RAPDs could be mapped, but they were too loosely linked at recombinant frequency near 50 % to warrant serious consideration. The availability of a genetic linkage map in Pinus sylvestris with a large number of RAPD markers should facilitate the identification of quantitative trait loci, contribute towards marker-assisted selection and allow the choice of unlinked RAPD markers for population genetic studies
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