Extraction of ATPase amyloplast envelope membranes of sycamore
1991
Poontariga Harinasut (Kasetsart Univ., Bangkok (Thailand). Faculty of Science. Dept. of Biochemistry)
Objective of this study was investigation of suitable detergents for Mg2+-ATPase extraction from the amyloplast envelope membranes of suspension-cultured cell of sycamore (Acer pseudoplatanus L.) Among four types of detergents tested: Triton X-100, octylglucoside, sarkosinate and deoxycholate, Triton X-100 at the proportion Triton: membrane protein weight = 3:1, is suitable for ATPase-extraction. The solubilizable fraction was applied on sucrose density gradient. After centrifugation, the fraction with maximum ATPase activity possesses a predominant polypeptide of 37-kDa. The result of the autoradiographic technique using adenosine triphosphotyridoxal suggested that the 37-kDa polypeptide may be a subunit of the Mg2+-ATPase of amyloplast envelope membranes.
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