The effect of pulsed excimer laser radiation on the contamination,growth and subsequent development of golden pothos (Epipremnum aureumL.)in vitro
1994
Al-Juboory, K.H. (Baghdad Univ. Coll. of Agric. (Iraq)) | Wiliams, D.J. | Nayfeh, M.H. | Skirvin, R.M. (Illinois Univ. Urbana,IL 61801,(USA))
Shoots of greenhouse-grown golden pothos (Epipremnum aureum L.) were surface disinfested and explanted on modified Murashige and Skoog (MS) medium. Later they were treated with pulsed XeCl excimer laser radiation showed significantly less contamination (23 and 10 respectively, versus 75 for control). In addition,we demon strated that pulsed XeCl excimer laser radiation affected the subsequent growth of callus,shoot proliferation,and leaf size of in vitro plants. The reason for this increased growth needs further investigation. Both benzyladeaine and thidiazuron were important for increasing the number of shoots generated from a single-shoot explant as well as inducing adventitions shoot organogenesis from pothos callus. Good rooting occurred on MS medium with 10 micromol NAA without TDZ or BA of the 50 rooted ex vitro plants from experiment 1,only 15 were variegated like the periclinal chimeral parental clone. The others were either pure green or albino,suggesting chimeral segregation
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