Stable, convenient-working extractants for the determination of L-ascorbic acid in citrus extracts
1996
Widodo, S.E. (Kyushu Univ., Fukuoka (Japan). Faculty of Agriculture) | Shiraishi, M. | Shiraishi, S.
As an effort to find a more stable, convenient-working extractant than regularly used methaphosphoric acid for determination of L-ascorbic acid (AA) in citrus extracts, several extractants for assay of AA were examined. The extractants tested were (I) 15 g stick HPO3 + 40 ml acetate + 450ml H2O, (II) 0.5% stick HPO3 + 0.2% Oxalate + H2O, (III) 0.2% Oxalate + 5% Acetate + A2O, (IV) 0.2% Oxalate + 10% Acetate + H2O, (V) 7% Citrate + 0.5% Malate + H2O. Based on measurements of AA stored in the extractants and AA content in the juice of Citrus limon Burm. cv. Frost Nucellar Lisbon, the combinations of oxalate plus acetate resulted in more convenient, less expensive and more stable extractants
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