Effect of cellulase preparation originated from Acremonium cellulolyticus Y-94 on alfalfa [Medicago sativa] silage fermentation
1996
Tomoda, Y. (Meiji Seika Kaisha Ltd., Sakado, Saitama (Japan). Bio Science Labs.) | Ohmomo, S. | Tanaka, O. | Kitamoto, H. | Hamaya, T. | Kono, T. | Tanno, Y.
Alfalfa was harvested (forth cutting in 1991 and first to forth cutting in 1993) and wilted overnight, then chopped into 2 cm lengths. The forage mixed with cellulases (9-1.0% of the forage) or glucose (1%), and lactic acid bacteria (0.001%) was tightly packed in 1 liter experimental silos or in nylon/polyethylene bagsilos. Both the silos were kept at 26 degrees C for 30 and 14 days, respectively. Each ensiling had three replications. Treatment of alfalfa from the forth cutting in 1991 with cellulase preparation originated from Acremonium cellulolytius Y-94 (ACS2) at 0.01% resulted in a lower pH, a higher lactic acid content, and a higher L/T value than obtained using a cellulase preparation from Trichoderma viride (CEP, 0.01%), or 1% glucose. Also, in the case of alfalfa from the fist through to forth cuttings of 1993, the addition of ACS2 and lactic acid bacterial promoted the fermentation and much improved the quality of silage. These results showed that the addition of 0.01% ACS2 and lactic acid bacteria was enough to improve the fermentation quality of alfalfa silage
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