Cloning and characterization of a chitosanase gene from the plant pathogenic fungus Fusarium solani
1996
Shimosaka, M. (Shinshu Univ., Ueda, Nagano (Japan). Faculty of Textile Science) | Kumehara, M. | Zhang, X.Y. | Nogawa, M. | Okazaki, M.
The plant pathogenic fungus Fusarium solani F. sp. phaseoli SUF386 secretes a chitosanase in the absence of exogenous chitosan. Based on partial amino acid sequences of the purified chitosanase, two degenerate oligonucleotides were synthesized and used as reverse transcription-mediated PCR (RT-PCR) primers to amplify a 500-bp fragment of corresponding cDNA. The PCR product was used as a probe to isolate the genomic copy of the gene (csn). F. solani csn has an open reading frame encoding a polypoptide of 304 amino acid residues with a calculated molecular mass of 31,876 Da and containing a putative 19-amino acid reside signal sequence. comparison between the genomic and cDNA sequences revealed that three introns are present in the coding region
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