Production of commercial Lilium rubellum Baker bulbs: Effects of volume and renewal of liquid medium on in vitro growth, bulb rot infection during cold treatment, and post-in-vitro growth of bulblets
1997
Niimi, Y. (Niigata Univ. (Japan). Faculty of Agriculture) | Nakano, M. | Saito, S.
Bulblets of Lilium rubellum Baker were cultured for 16 weeks in a 20-, 30-, or 40-ml liquid medium renewed 9, 1, or 3 times. The bulblets were assessed for growth and rot infection. (1) Bulblets with fresh weight of more than 600 mg were obtained by using a 20- or 30-mg medium with 3 renewals, or 40 ml with one renewal. (2) Bulblets frequently turned brown when they were cultured in a 30- or 40-ml medium with 3 renewals; they frequently rotted both during a cold treatment and after transplantation. (3) Bulblets cultured in a 20-ml medium did not rot after a cold treatment irrespective of the number of medium renewals; they grew well after transplantation to the greenhouse. (4) Bulblets cultured in a 20-ml medium with 3 renewals most frequently developed into plantlets with elongated axes 15 weeks after transplantation. These results indicate that the 20-ml medium with 3 renewals appears to be most suitable for bulblet culture of L. rubellum in a liquid medium
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