Quantification of NADH-cytochrome b5 reductase (metmyoglobin-reducing enzyme) in bovine skeletal muscle by an immunoblotting assay
1997
Arihara, K. (Kitasato Univ., Towada, Aomori (Japan). Coll. of Veterinary and Animal Sciences) | Itoh, M. | Kondo, Y.
Quantitative determination method of NADH cytochrome b5 reductase (metmyoglobin-reducing enzyme) in bovine skeletal muscle was developed by combining SDS-PAGE and immunoblotting techniques. Recoveries of the enzyme by this assay ranged from 90.0 to 94.4%. The content of NADH-cytochrome b5 reductase in bovine skeletal muscle was estimated to be 13.8 +- 2.6 (9.0-18.2) mu-g/g tissue (n=19). Also, a quantitative assay for determining cytochrome b5 (an electron transfer mediator for metmyoglobin reduction) was similarly established and cytochrome b5 content in bovine skeletal muscle was estimated to be 59.0 +- 20.9 mu-g/g tissue. The quantitative data of NADH-cytochrome b5 reductase in this study supported our previous hypothesis that the enzyme plays a critical role in preventing metmyoglobin accumulation if muscles
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