Feeding deterrent activity of alkaloids from yam, Dioscorea hispida Schlussel (Dioscoreaceae) on larvae of diamondback moth, Plutella xylostella L. (Lepidoptera: Yponomeutidae)
1996
Banaag, A.B. (Mindanao State Univ. 9200 Iligan City (Philippines). Iligan Inst. of Technology)
Dioscorea hispida Schlussel rhizomes (nami) was extracted with methanol and the extract was further separated to obtain alkaloid fractions by column chromatography and thin layer chromatography. A leaf disk bioassay was employed to evaluate feeding deterrent activity against the diamondback moth (DBM) larvae, Plutella xylostella L. Alkaloid fraction showed strong feeding deterrent activity to DBM larvae. Two alkaloids A and B were separated as active components. Alkaloids A was more active than alkaloid B at 100 to 250 ug/ml, and a binary mixture of these two alkaloids also inhibited larval feeding at lower concentrations (50 to 100 ug/ml) than either alkaloid separately
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