Interaction of Rhizobium sp. to 32 mungbean lines
1992
Sawai Pongkao | Rangsarid Kaveeta (Kasetsart Univ., Bangkok (Thailand). Dept. of Agronomy.)
The 32 selected mungbean lines (Vigna radiata) derived from the Mungbean Breeding Project and the standard cultivar, Uthong-1, were grown for study of the response to rhizobium in two inoculated conditions: inoculation and no-inoculation at Kasetsart University, Kamphaengsaen Campus. The non-replicated design was employed due to the inadequacy of seeds. Mixed 15-15-15 fertilizer was applied at the rate of 25 kg/rai. Seeds were drilled in the rows and immediately followed by powdered rhizobial inoculum sprinkled over the seeds at the rate of 400 g/rai. After germination, seedlings were thinned to 10 plants per linear metre giving a target population of 32,000 plants/rai. At full bloom or the beginning of young pod formation, 10 plants at random from the two middle rows were fug for observation on nodule formation on the tap and lateral roots. Large size nodules (not less than 0.1 cm in diameter) whether formed on the tap or lateral roots were recorded. Then, 10 randomed nodules from each type of roots were cut to identify whether as inefficient of efficient nodules. Efficient nodules had pink-red interior. Some other characteristics, yield and yield components were also recorded. In average nodules on branch roots were about 3 to 4 folds more than on tap root of the same plant in almost every lines tested. Every lines responded differently to the nodule formation, inoculated treatment was superior to the no inoculation either no quantity and efficiency. The presence of nodules on the no inoculation treatment indicated that natural population of rhizobia or population of rhizobia from previous crops were present in the soil. Lines that were well nodulated and produced efficient nodules with relatively high yield whether inoculated or not inoculated were as follows: VC 2742-36-3B, VC 2742-38-3B, VC 2745-8-3B, VC 2745-9-3B and VC 2745-20-3B.
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