Efficacy of oxolinic acid against vibriosis infection in black tiger shrimp (Penaeus monodon Fabricius)
1994
Thanathip Lamkom
Efficacy of oxolinic acid against Vibrio alginolyticus infection in black tiger shrimp (Penaeus monodon) was studied. Biochemical characteristics of V. alginolyticus isolated from diseased shrimp was studied. This species could utilize arabinose, sucrose, saccharose and produced lysine and ornithine decarboxylase enzyme. Yellow colony on TCBS agar and growth in media 10 % with NaCl were observed. Shrimps (averaging 10 gm) were fed with medicated pellet feed (2.5 g per kg of feed) for three days before intramuscular injection with V. alginolyticus at a dosage of LD50 level (5.1 * 10 ** (6) cells) and survival rate was observed for seven days. Shrimp fed with medicated feed had higher survival rate (73.33 %) than control (34.67 %). Histopathological changes in shrimp injected intramuscularly with this bacteria was studied. At 3 hours post injection, the saline injected controls showed the least damaged tissue. The non - pre medicated feed group showed more extensive damaged tissue with small nodules formation than shrimp fed with medicated feed. Seven days post injection, an affected area in the control group was nearly healed whereas large damaged area with dense haemocytes were found in bacterial injected shrimp. The damaged site in non - treated shrimp was larger than in pre - treated one and healed slower.
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