(Evaluation of different ELISA procedures for the detection of pea seed-borne mosaic potyvirus and broad bean stain comovirus in lentil leaf extracts)
1993
Kumari, S.G. | Makkouk, K.M.
Local lentil isolates of pea seed-borne mosaic potyvirus (PSbMV) and broad bean stain comovirus (BBSV) from Syria were purified. The purified preparations were injected into rabbits and antisera with good quality were obtained. These antisera were used in a comparative study to evaluate five different ELISA procedures for the detection of PSbMV and BBSV in infected lentil leaf extracts. In each test twenty dilutions (from 1/10 until 1/5,242,880) were tested. The most sensitive procedure was ELISA with enzyme amplification (EA-ELISA), where BBSV was detected in sap dilution of 1/2,621,440 and PSbMV in sap dilution of 1/40,960, followed by penicillinase ELISA (PNC-ELISA), indirect ELISA (I-ELISA) and double antibody sandwish ELISA (DAS-ELISA). Dot-blot ELISA was the least sensitive, but was able to detect BBSV in sap dillution of 1/20,480 and PSbMV in 1/2,540.
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