Optimum time and medium for in vitro capacitation of buffalo spermatozoa
1996
Dhaka, S.P. | Dhanda, O.P. (CCS Haryana Agricultural Univ., Hisar - 125 004, Haryana (India). Dept. of Animal Production Physiology)
A study was conducted to find out the best medium and the optimum period of incubation for the capacitation of buffalo spermatozoa in vitro. Ten ejaculates from each of four Murrah bulls (5-6 years old) were subjected to four different media, i.e., TCM-199; TCM-199 + calcium ionophore (0.5 micro M); TCM-199 + heparin (10 microgram/ml); or uterine fluid for a period of 5, 8, and 10 h, respectively. The mean proportions of capacitated spermatozoa in various media were found to be 70, 85, 86, and 69 percent, respectively. Addition of heparin to TCM-199 was observed to provide the maximum degree of capacitation followed by TCM-199 + calcium ionophore. TCM-199 or the uterine fluid provided similar success of capacitation, although lower capacitation percentage than the two other media. The capacitation at 5, 8, and 10 h was observed to be 70, 79, and 83 percent, respectively. Sperm motility decreased progressively from 92 percent initial motility to 32 percent after ten hours of incubation in all the bulls. The various media and the incubation period differed significantly (P0.05) from each other with respect to the degree of capacitation. It was concluded that buffalo spermatozoa are capable of acquiring good degree of acrosome reaction and capacitation when incubated in uterine fluid for a period of five hours
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