Effect of NAA and BAP on callus culture and plant regeneration in curly kale (Brassica oleraces L.)
1999
Ahmad, S. (National Agricultural Research Centre, Islamabad (Pakistan). Seed Science and Technology Programme) | Spoor, W.
The effect of growth regulators on Curly kale (Brassica oleraces L.) was studied in the Plant Growth Unit and Laboratories at Scottish Agriculture College, University of Edinburgh. Concentrations of NAA (Naphthalene acetic acid) and BAP (6-Benzyl Amino Purine) individually as well as in combination had different effects on different stages of tissue culture. In general, when ever NAA and BAP were in balance (i.e. NAA/BAP = 1), callus growth was enhanced. Media supplemented with more concentration of BAP with no or low levels of NAA promoted shoot formation and reverse media type initiated roots. Maximum callus growth was observed on media supplemented with 1-1.5 mg l-1 each of NAA and BAP, while media supplemented with 1 mg l-1 of BAP only, showed best regeneration.
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