Antibacterial property of the malic acid from the leaves of Katakataka (Kalanchoe pinnata, Lam., Fam. Crassulaceae)
1995
Jazul, R.A. (Centro Escolar Univ., Manila (Philippines). Graduate School)
The purpose of the study was to determine the antibacterial property of the malic acid from the leaves of a native plant Katakataka (Kalanchoe pinnata, Lam. Fam. Crasseulaceae). The herb katakataka is abundant in the countryside and was reputed among the rural folk as medicinal. The plant is easily propagated through the little plantlets along the margin of the leaf. Acid content of the leaves were determined and subsequently subjected the malic acid content to analysis by Thin Layer Chromatography (TLC) and infrared spectroscopy. The cylinder plate assay method was used for microbiological testing of the macid acid extractive. The test organisms used were Staphylococcus aureus, Escherichia coli, Enterobacter aerogenes, and Candida albicans. The antibacterial standard used was ampicillin trihydrate for the bacteria and miconazole nitrate for the fungi. The zones of inhibition were observed to determine the activity on specific organisms. The major findings of the study were: 1.) the organic acid extract from katakataka which was obtained by employing decoction method, contained 40.07 percent pure malic acid; 2.) both the TLC and the infrared spectrum showed that the plant is indeed a source of malic acid; 3.) the microbiological test results showed that the katakataka malic acid extract is effective against bacteria but not against fungi
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