[NPV and neem extract in Heiothis armigera control]
1990
Tipvadee Attathom | Kwanchai Sombatsiri | Chalit Mahatthanaat (Kasetsart Univ., Bangkok (Thailand). Faculty of Agriculture. Dept of Entomology)
Nuclear polyhedrosis virus used in this study was isolated from diseased Heliothis armigera larva found in Thailand. Attempts were made to mass propagate this virus in the laboratory using bollworm as the insect host. Based on the percent mortality and virus yield, the results indicated that the optimum virus inoculum size was 2.5*10*[5) PIBs/larva and the most suitable stage of insect for virus production was the 3rd instar larva. The incorporation of adjuvants with insect virus was experimented in attempts to improve virus efficacy. Several materials that acted as UV protectant, insect attractants, suspending agent, dilulents and wetting agent were mixed with the virus and bioassayed against H. armigera larvae. Charcoal, milk powder, molasses soybean oil, soybean flour and cottonseed flour had high potentiality to be used as adjuvants for virus formulation. Field trials of some virus formulations in controlling the bollworm species were under investigation.
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