Biological properties of ram spermatozoa frozen in pellets and thawed in a sugar-lactose citrate diluent
1999
Zakhariev, Z. | Al-Khanak, Kh. (Institut po Biologiya i Imunologiya na Razmnozhavaneto i Razvitie na Organizmite - B"lgarska Akademiya na Naukite, Sofia (Bulgaria)) | Georgiev, G. | Anev, G.
Sixty eight ewes at spontaneous heat were insemineted intrauterinely with frozen-thawed semen by using Karl Storz endoscope. A new sugar-lactose citrate diluent (21) was used for thawing and in vitro storage of semen. Fertility rate following intrauterine insemination was 58.8%. Results indicate that the diluent guarantees good protection of biological activity of frozen-thawed ram spermatozoa and can be applied in Al.
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