The reaction of test-tube plants on the potato late blight pathogene - Phytophthora infestans (Mont.) d BY
2000
Rosenberg, V. | Talvoja, P. | Kusma, E. (Estonian Agricultural Univ., Tartu (Estonia))
The test-tube-plant with the fungus Phytophthora infestans creates in the test-tube an association, which serves as a model for the research on the connections between the host-plant and the pathogene. In case of the inoculation with the suspension of the pathogene spores, the disease symptoms in the plant will be revealed in 2-5 days in the form of sporulation. After that, the necrosis of the sells of the host-plant at the place of sporulation will take more days and its duration (in hours) will be an evidence of the general resistance of the cultivar. The pathogene grows on the agar for frowing potato plants, but it will form sporangiospores in the test-tube, growing together with the plant. In addition to that, on the agar surface in the test-tube the fungus Ph. infestans will form a cotton-wool-like lump, which will be the larger, the more susceptible to the disease a cultivar is. On the cultivar, which is protected by the resistance-genes (variety 'Sarme'), the fungus-lump will not be formed, but on the agar surface around the plant roots, there may be observed single sporangiospores of the fungus, or a coating by the fungus, which may be seen by the naked eye. Under the same conditions, one part of the plants in the fungus-lump will completely survive, while the other plant will perish. These plants that survive, may be the carries of the acquired immunity
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