Identification of Drechslera graminea isolates by cultural characters and RAPD analysis
2000
Jawhar, M. | Sangwan, R.S. | Arabi, M.I.E.
Isolates of Drechslera graminea, the causal agent of barley leaf stripe, were collected from different regions of Syria and analyzed for differences in cultural morphology, growth rate, colony colour, and randomly applied polymorphic DNA (RAPD) markers. Differences in morphological characters were detected among the isolates used. Molecular polymorphisms (RAPDs) were also detected among them. However only two out of the twelve random primers used OPK3 (5'-CCAGCTTAGG-3') and OPK5 (5'-TCTGTCGAGG-3') revealed a different number and size of amplification bands between isolates. These two primers were sufficient to identify most of the isolates studied.
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