Effect of concomitant ovule culture on anther culturability in wheat and wheat x wheatgrass wide crosses
1999
Sharma, H. | Jekkel, Z. | Benlhabib, O. | Ohm, H.
Anther culture is a useful method to produce doubled haploids from pollen. It also holds potential in producing addition, substition and translocation lines from wide crosses. However, low callus induction and green plantlet regeneration limit the routine application of anther culture in wheat. In an effort to improve anther culturability, the effect of co-culture of ovules with anthers on solid media was studied in six wheat genotypes and three wheat x wheatgrass backcrosses. Wheat genotypes differed significantly for callus induction as well as plantlet regeneration and the trend remained the same in anther culture as well as in co-culture.
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