[Effect of protein sources and carbohydrate with different rumen degradation level on in vitro rumen degradation]
1999
Rossi, E.
The objective of this work was to study the effect of combination protein/N (CP) and carbohydrate (CH) sources with varying by-pass protein on in-vitro rumen degradation of dry matter (DM), organic matter (OM) and crude protein (CP); characteristic rumen fermentation. Rumen fluid was taken from a fistulated cattle, fed 70 percent roughage : 30 concentrate diet. 40 mg CP from urea, soybean meal (SBM) or blood meal (BM) were fermented with 140 mg total digestible nutrient (TDN) from rice bran (RB), sago waste (SW) or tapioka flour (TF) for 24 in inoculum (2.5 : 1.5, Mc Dougllas Buffer : rumen fluid; three runs, 2 tubes x treatment-1 x run-1). The result showed that effects of protein sources with varying by-pass protein level on DM, OM degradation were highly significant different (P less than 0.01). There were interactions (P less than 0.01) between CP and CH sources on CP degradation, concentration of NH3 and total volatile fatty acid (VFA). However, no responses (P more than 0.05) were observed on pH rumen fluid. In conclusion, DM and OM rumen degradation were increased by increasing CP degradation of feedstuff. Degradation of CP and concentration of NH3 and total VFA were affected by combination protein/N and energy sources with varying by-pass protein
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