Further serological characterization of two tobravirus isolates from Algeria and Libya
1998
Makkouk, K.M. | Kumari, S.G. (International Centre for Agricultural Research in the Dry Areas, Aleppo (Syria). Germplasm Programme)
Antisera against purified particles of tobravirus isolates from Libya (LYV 66-91) and Algeria (AlgRI 0) were produced and used for their serological comparison with other isolates of pea early-browning tobravirus (PEBV), including one of the broad bean yellow band serotype (PEBV:BBYB) and with the PRN strain of tobacco rattle tobravirus (TRV:PRN). DASELISA, DAC-ELISA and dot-blot BLISA showed that the Algerian and the Libyan isolates represent two different serotype. In DAS-BLISA, the Libyan isolate was similar to the Dutch isolate of PEBV (isolate EI 16). In DAC-ELISA, the Algerian isolate reacted strongly with PEBV:BBYB and the TRV:PRN antibodies but not with those to the Libyan or the Dutch isolates of PBBV. Using dot-blot ELISA, the Libyan isolate was serologically closely related to the Dutch isolate of PBBV and distinct from the Algerian isolate of PEBV, PEBV:BBYB and TRV:PRN.
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