Lupin spp responses to Fusarium oxysporum f. sp. lupini and Fusarium moniliforme
1999
Jorge-Silva, M.L. | Queiroz, A. | Pedroso, E.I. | Melo, E.M.P.F. | Veloso, M.M.
Lupins are important grain legumes that can be grown in several Portuguese regions (e.g. "Beira Interior") but susceptibility to Fusarium may cause problems. We are analysing in this work the responses of Lupinus albus to infection by F. oxysporum f. sp. lupini and F. moniliforme. Active defense in plants is associated with marked metabolic alterations, but little is known about the exact role of the reported changes in specific activity of several enzymes and proteins (particularly PR proteins) in infected plants. Leaf proteins were separated by SDS-PAGE, a few polypeptides being accumulated as a consequence of the infections. To understand the relationships between fungal growth and the accumulation of chitinase and a thaumathin-like protein we used two antisera produced against these proteins from L. albus. A higher increase in the thaumathin-like protein compared to chitinase was observed. We have also studied, histologically, with the optical microscope, the distribution of the proteins in Lupinus root tissues healthy and infected with F. oxysporum f. sp. lupini. Marked alterations in the chitinase level were detected in the root stele. The scanning electron microscope reveals the presence of fungal hiphae inside the xylem vessels
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