Protein profiles in roots of aluminium sensitive and resistant barley cultivars after aluminium treatment
1999
Tamas, L. | Mistrik, I. | Hutova, J. (Slovak Academy of Sciences, Bratislava (Slovak Republic). Institute of Botany)
Alterations of protein composition in Al-sensitive and resistant barley cultivars were examined in extracellular and intracellular compartments of the roots after Al stress. The greates changes in protein composition were found in cell wall fraction of the roots. Exposure of roots to 100 micro mmol/L Al resulted in a reduction of several proteins in both Al-sensitive and Al-resistant plants. However, at the same time the synthesis of six different proteins was stimulated. Two of them with molecular mass of 14 and 16 kDa accumulated only in the resistant cultivar, while the other four proteins which were accumulated in both Al-sensitive and Al-resistant cultivars. Within the peripheral membrane proteins Al-induced reduction of small polypeptides was observed. Content of only two proteins increased in both cultivars following Al exposure, while accumulation of four proteins was specific for the Al-sensitive cultivar. In the fraction of integral membrane proteins, Al stress induced accumulation of four proteins, three of which were accumulated both in sensitive and resistant cultivars. The 40 kDa polypeptide, observed after Al treatment in both cultivars, has also been presented in control plants of resistant cultivar. Analysis of cytoplasmic proteins revealed a very intensive Al induced accumulation of 28 kDa polypeptide in root of both Al-sensitive and Al-resistant barley cultivars. The observed differences in polypeptide pattern between control and Al-treated roots indicate the role of proteins in the response of barley plants to aluminium stress
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