Circadian oscillations of activities of rat liver adaptive enzymes during a short-term feed deprivation
2000
Toropila, M. | Ondrasovic, M. | Danko, J. | Falis, M. | Svicky, E. (University of Veterinary Medicine, Kosice (Slovak Republic))
After a 3-week period of adaptation to a standard artificial light regimen: light : darkness - 12 h : 12 h (07.00-19.00 h light, 19.00-07.00 h darkness), the experimental animals, male rats of Wistar strain SPF breeding, were deprived of feed starting at 08.00 h. During the subsequent 48 h (animals were deprived of feed for 0 - 48 h), activities of selected adaptive enzymes, tyrosine aminotransferase (TAT), tryptophan-2-3-dioxygenase (TO), aspartate aminotransferase (AST), and alanine aminotransferase (ALT) were determined in the liver cytosol fraction at 3-h intervals.Although the rhythm of circadian oscillations of selected enzyme activities was preserved in the animals fasting for different periods, some characteristics of the activities changed in comparison with well-fed animals, particularly during the dark period of the day
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