Histological studies on the ovaries of adult giant freshwater prawns, Macrobrachium rosenbergii (de Man)
1993
Punnipa Ariyakulkaln | Phuntipa Kittisophon | Wandee Poolsanguan
Histology and histochemical studies on the ovaries of adult Macrobrachium rosenbergii at various reproductive states were reported. The histochemical staining with periodic acid-Schiff, alcian blue and oil-red-O demonstrated the presence of glycoproteins and lipids in primary and secondary vitellogenic oocytes as well as in ripe eggs, but the absence in oogonia and previtellogenic oocytes. Paraffin sections stained with H/E of ovaries on 2nd-12th days after a regular molt contained rows of developing oocytes starting from oogonia to secondary vitellogenic oocytes. They became larger, approaching maturation, on the 28th day. After a premating molt, the ovaries were remarkably large with orange color and were occupied by fully mature oocytes of either ovoid of hexagonal shape. Ovaries became thin and pale after spawning. There were empty trabeculae of connective tissues with layers of follicle cells and clusters of oogonia and previtellogenic oocytes at the center. The amount of oocytes increased with time. From the 13th day postspawning onward, two types of ovaries were identified: the small yellow ovaries with developing oocytes from oogonia to primary vitellogenesis and the larger orange ovaries with maturing oocytes. The maturation of oocytes were continued on 19th day to 28th day postspawning. Different sizes of ripe eggs among individuals were observed.
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