Control of stem end rot of mango by yeasts
1997
Montatip Sourha
Inoculation of mycelium disc on mango fruits nearby the stem end indicated that the mycelium, Lasiodiplodia theobromae had grown directly toward to the stem end and penetrated through stem end tissues. For conidial inoculation at the stem end, the conidia could germinate and penetrate germ tube through the wound at stem end tissues. The disease symptom on mango fruit appeared while the masses of mycelium penetrated through the stem end tissues. Twelve yeasts isolates were investigated for their ability in inhibiting the mycelial growth of L. theobromae, Rhodotorula sp. were the best and Hansenula saturnus respectively. Spraying the fungal conidial suspension before and after the yeast suspension spraying 1 hour, and spraying fungal conidial suspension and yeast suspension at the same time found that their ability in inhibiting the fungal conidia growth were non significant. Screening of 12 yeasts to control fruit rot on banana fruits (L. theobromae) found that Pichia membranaefaciens was the best, then Candida tropicalis and Hansenula saturnus respectively. Within three selected yeasts : C. tropicalis, H. saturnus, and Saccharomyces cerevisiae found that C. tropicalis were a good control of stem end rot of mango fruits. The yeasts showed no antibiotic production on PDA against L. theobromae. C. tropicalis effected on conidia gemination of L. theobromae. Treated mango fruits with C. tropicalis and kept under modified atmosphere by wrapping with plastic film (M Wrap), could reduce stem end rot caused by L. theobromae by 26.67 percent.
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