Etiology and symptomatology of grassy shoot syndrome of sugarcane
1997
Upsorn Pliansinchai | Nonglak Sarindu | Siriphat Prammanee (Suphan Buri Field Crops Research Center (Thailand))
Etiology was studied to detect the causal organism of sugarcane grassy shoot-like syndrome in Thailand. Electron microscopy revealed mycoplasma-like organism in the ultrathin section of symptom plant. ELISA test with white leaf MLO antiserum showed either positive and negative. DNA was detected in grass shoot sample. It was remarkable that the extra DNA band was not normally found in dsRNA extraction. So it is possible to be DNA from the causal agent rather then DNA from the host. The result suggested that MLO possibly associated with sugarcane grassy-shoot like syndrome. It was also shown that the disease could transmitted through cane setts of U-thong 1 variety upto 87.5 percent. In severely infected cane when planted, symptom appearred 2 month after planting, whereas, in mild infected cane, symptom appeared 5 months after planting. Symptom expression became more severe in ratoon result in grass-like symptom and a few millable cane were produced. Incidence were upto 97.3 and 76.9 percent in ratoon when severely infected and mild infected cane were planted. The result suggested that selection of seed cane and seed piece treatment could possibly control this disease.
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