Chloroplast DNA polymorphism in genus Vigna
1994
Somjit Tinkrathok
Analysis of chloroplast DNA fragment polymorphism is one method for studying the evolutionary relationship among plants. In this study, 23 accessions from 5 species of Vigna i.e. V. umbellata, V. angularis, V. unguiculata, V. radiata and V. mungo collected in Thailand were used. Cholorplast DNA was extracted from 20 to 25-day-old plant. Chloroplast were isolated from each sample and then lysed for DNA extraction. The DNA was digested with 7 restriction enzymes, Dral, EcoRI, EcoRV, HindIII, KpnI, PstI. and XhoI and analyzed by electrophoresis on 0.8 percent agarose gel. Upon comparing the pattern of restricted DNA from each accession, the plants were classified into 4 groups. The difference between V. angularis and V. unguiculata and the difference within species were not detected. The proportion of sheared fragments showed that V. radiata was closely related to V.mungo with only 9 percent difference. The most difference about 15 percent was found between V. radiata and V. angularis or V. unguiculata.
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