Acclimatization of wheat regenerants produced by in vitro anther culture
1996
Sesek, S. | Dencic, S. (Naucni institut za ratarstvo i povrtarstvo, Novi Sad (Yugoslavia))
An investigation was conducted to assess three methods of acclimatization and vernalization of winter wheat regenerants produced by in vitro anther culture. The first method involved the acclimatization of regenerants in a phytotron, in controlled conditions of relative humidity (95%), temperature (28 deg C) and light (2,00 lx), vernalization in a cold chamber at 4-5 deg C, and subsequent cultivation of regenerants in a greenhouse. The second method involved the acclimatization of regenerants at high humidity (covered with a foil) and the temperature of 26-28 deg C, vernalization in a cold chamber and subsequent cultivation in a greenhouse. The third method involved the acclimatization of regenerants at high humidity and the temperature of 26-28 deg C, vernalization in a cold chamber at 5-10 deg C and subsequent cultivation in natural conditions of the open field. The results showed that the third method produced the largest number of regenerants that reached the stage of physiological maturity - 90%. The plants matured in the first third of July. The second method produced 58% of mature regenerants which reached the stage of physiological maturity in the second or last third of May. Of the regenerants cultivated in the phytotron, 72% reached the stage of physiological maturity, in the last third of February or the first third of March.
Mostrar más [+] Menos [-]Palabras clave de AGROVOC
Información bibliográfica
Este registro bibliográfico ha sido proporcionado por Unassigned data from Yugoslavia