Plant regeneration from leaf tissues of apple cultivars Granny Smith, Morspur Golden and Starkrimson
2000
Gercheva, P. | Andinova, T. | Ivanova, K. (Fruit Growing Institute, BG-4004 Plovdiv (Bulgaria))
The influence of the nutrient media content (mileral salts, plant growth regulators, carbohydrates) and genotype on the regeneration from leaf segments of apple cultivars Granny Smith, Morspur Golden and Starkrimson was studied. Nutrien media based on MS macro and micro salts, 2,5mM IBA 7,5mM TDZ induced the best regenerative reply for all the cultivars. Optimal saccharose/sorbitol ratio for Morspur Golden (53% regeneration) and Granny Smith (87% regeneration) was found 29,2 mM and for Starkrimson (100% regeneration)-29,2mM/164,7mM. Differences in regenerative rate and callus formation percentage of the three cultivars were reported that probably were due to genome features.
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